Substrate: N-benzoyl-L-tyrosine ethyl ester (BTEE) 0.001M
Buffer:0.1M tris-HCl, pH 7.8, containing 0.1M CaCl₂.
Enzyme:1mg/ml chymotrypsin A in 0.001M HCI.
N.B. The enzyme provided is a stock solution which should be diluted 50-fold with
0.001M HCl immediately before use.

How do I do this fold dilution calculation ?

Answers

Answer 1

Based on the dilution formula, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.

How can 50-fold dilution of the enzyme be done?

The 50-fold dilution of the stock enzyme solution can be done by using the dilution formula to determine the given volume of the stock solution required.

The dilution formula is given below:

C1V1 = C2V2

where:

C1 = Initial concentration of enzymeC2 = Final concentration of enzymeV1 = Initial volumeV2 = Final volume

From the given data for the enzyme dilution;

C1 = 1

C2 = 1/50 = 0.02

V1 = x

V2 = 5 ml

Making V1 subject of formula in the dilution formula:

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.

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Related Questions

Does a displacement reaction take place in 'magnesium + lead nitrate'? and if so why?

Answers

Answer:

Maybe or maybe not (not sure)

Explanation:

A displacement reaction is a type of reaction where one element is displaced by another from a compound.

In the case of magnesium and lead nitrate, magnesium is more reactive than lead. Therefore, it will displace lead from lead nitrate to form magnesium nitrate and lead.

The reaction can be represented as:

Mg(s) + Pb(NO3)2(aq) → Mg(NO3)2(aq) + Pb(s)


Another answer could be;

A displacement reaction does not take place in 'magnesium + lead nitrate' because magnesium is more reactive than lead.

ASAP PLS 100 POINTS Which statement best describes the role of oxygen in cellular respiration?
A. Oxygen molecules are a waste product of electron flow.
OB. Oxygen molecules form as ATP molecules are made.
OC. Oxygen molecules form as sugar molecules are broken down.
OD. Oxygen molecules are used to make water molecules.

Answers

It’s A .. I think I hope this helps !

Answer:

Option C

Explanation:

Oxygen molecules form as sugar molecules are broken down.

Sugar or carbohydrates are known as glucose or sucrose and malltose i.e C6H12O6 etc.

So they are broken down by cellular respiration and forms Oxygen for cells

The parent element 42 19 K decays by beta decay. Which daughter element will
form?
O A. Ca
OB. 1 Ar
O C. C
OD. K

Answers

Answer: A

Explanation:

This means that the atomic number decreases by 1 but the mass remains constant, meaning the atomic number of the new element is 20. This corresponds to calcium.

an imaginary gas phase reaction, according to the equation below is carried out in a closed container. 3.5 moles of A and 3.5 moles of B is introduced in the container. Reaction is carried out at 31.70C and volume of the container is 9.3 L. Percent yield of this reaction is 86.8%. Calculate the pressure of the container after reaction took place. R =0.0821 L.atm/mol.K
A+ 2B → AB2

Answers

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True or False?
The same carbon atoms are used repeatedly on Earth.

Answers

Answer:

True

Explanation:

had the same question

If a penny has 0.035 g of copper(I)oxide coating its surface, what volume of 0.1 M acetic acid should be
used to clean it?

Answers

We need 8.8 mL of acetic acid
Explanation:
Step 1: Data given
Mass of Copper oxide (CuO) = 0.035 grams
Molar mass CuO = 79.545 g/mol
Molarity acetic acid = 0.10 M
Step 2: The balanced equation
CuO + 2CH3COOH → Cu(CH3COO)2 + H2O
Step 3: Calculate moles CuO
Moles CuO = mass CuO / molar mass CuO
Moles CuO = 0.035 grams / 79.545 g/mol
Moles CuO = 4.4 *10^-4 moles
Step 4: Calculate moles CH3COOH
For 1 mol CuO we need 2 moles CH3COOH to produce 1 mol Cu(CH3COO)2 and 1 mol H2O
For 4.4 *10^-4 moles CuO we'll have 2 * 4.4 * 10^-4 = 8.8 *10^-4 moles
Step 5: Calculate volume of acetic acid
Volume acetic acid = moles acetic acid / molarity
Volume acetic acid = 8.8 * 10^-4 moles / 0.10 M
Volume acetic acid = 0.0088 L = 8.8 mL
We need 8.8 mL of acetic acid

please please helpppp
if someone could give me the answer on how to balance this equation with explanation that would be great.


Ag2O → Ag + 0₂

thanks in advance​

Answers

Answer: 2Ag2O -> 4Ag + O2

Explanation: The reason why we balance is to make sure that the number of atoms are conserved on both sides. Since the reactants side has 2 silver atoms and the product side only has one, we can tell that the equation isn’t balanced. It is also observable that oxygen isn’t balanced. We can just trial and error. We can start by adding a coefficient of 2 to Ag2O and see if we can balance the products side. By adding a 4 to the Ag the chemical equation is balanced. Both sides has 4 Ag atoms and 2 oxygen atoms

what is the difference between s8 and 8 s

Answers

the difference between them is s8 is a molecule of eight sulfur atoms covalently bonded together and 8s is eight separate sulfur atoms.

The weight of an object ____.
a) depends on its location
b) is not affected by gravity
c) is the same as its mass
d) is always the same

Answers

Explanation:

the. correct option is d

Which of the following stores chemical energy?

A. the bonds of a molecule

B. the nucleus of an atom

C. the density of a substance

D. the temperature of a substance

Answers

Answer:

A: The bonds of a Molecule

Explanation:

A=the bonds of a molecule

How does the entropy change in the reaction 2C3H6(g) + 902(g) → 6CO2(g) +
6 H₂O(g)?
OA. It is impossible to tell.
B. The entropy increases.
C. The entropy is constant.
D. The entropy decreases.

Answers

Taking into account the definition of entropy, the entropy increases (option B).

What is Entropy

Entropy can be interpreted as a measure of the random distribution of a system. In other words, the entropy S is a state function that measures the degree of molecular disorder of systems.

In this way, the change in entropy shows the change in molecular order that occurs in a chemical reaction. If the increase in entropy is positive, the products have a higher molecular disorder (higher entropy) than the reactants. On the other hand, when the increase is negative, the products are more ordered.

So, the entropy increases in the reaction if the total number of product molecules are greater than the total number of reactant molecules.

Entropy change in this case

In this case, there are 12 moles of gaseous product, and 11 moles of gaseous reactant in the equation. This is, there are more moles in the products than in the reactants.

Therefore, the entropy increases (option B).

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20.0 g of bromic acid, HBrO3, is reacted with excess HBr.

HBrO3 (aq) + 5 HBr (aq) → 3 H2O (l) + 3 Br2 (aq)

If 47.3 g of Br2 is produced, what is the percentage yield of Br2? Round your answer to the nearest whole percent.

Answers

After Rounding off The percentage yield is 64%

What is Percentage Yield ?

It is the ratio of actual yield to theoretical yield multiplied by 100% .

It is given in the question

20.0 g of bromic acid, HBrO3, is reacted with excess HBr.

The reaction is

HBrO₃ (aq) + 5 HBr (aq) → 3 H₂O (l) + 3 Br₂ (aq)

Actual yield = 47.3 grams

Molecular weight of Bromic Acid is 128.91 gram

Moles of Bromic Acid = 20/128.91 = 0.155 mole

Mole fraction ratio of Bromic Acid to Bromine is 1 :3

Therefore for 0.155 mole of Bromic Acid 3 * 0.155 = 0.465 mole of Bromine is produced.

1 mole of Bromine = 159.8 grams of Bromine

0.465 of Bromine = 74.31 grams of Bromine

Percentage Yield = (47.3/74.31)*100 = 63.65 %

After Rounding off The percentage yield is 64% .

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How many moles are in 2.372e+02 mg (milligrams) of bromic acid?

Answers

Answer:

1.840 x 10⁻³ mol HBrO₃

Explanation:

To find the moles of bromic acid (HBrO₃), you should (1) convert milligrams to grams (by dividing by 1,000) and then (2) convert moles to grams (via molar mass from periodic table).

Molar Mass (HBrO₃): 1.008 g/mol + 79.904 g/mol + 3(15.998 g/mol)

Molar Mass (HBrO₃): 128.906 g/mol

2.372 x 10² mg HBrO₃         1 g                1 mole
---------------------------------x----------------x------------------  = 1.840 x 10⁻³ mol HBrO₃
                                         1,000 mg      128.906 g

For an atom electrons, how many energy sublevels are present in the principal energy level n = 4?

Answers

There are 4 energy sublevels are present in the principal energy level n = 4.

What is sublevels?

Sub-level is a type of level that is lower than another level or it is a part or component of a big level. S, p, d and f are the four energy levels in which electrons are present.

So we can conclude that 4 energy sublevels are present in the principal energy level n = 4.

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8B-Zisette, Jennifer
ping
Complete the sentence to describe what a wave is and what it does.
A wave is a
that carries
from one place to another.

Answers

Answer:

Disturbance; energy.

Explanation:

A wave is a disturbance that transfers energy from one place to another without transferring matter.

2 HCl (g)
H2 (8)
Cl2 (8)
Is the model a correct representation of a chemical reaction taking place?
No, a chemical reaction always has more products than reactants.
Yes, the model shows atoms rearranging to produce a new compound.
No, a chemical reaction always produces more complex compounds.
Yes, the model shows atoms changing states of matter to form a product.

Answers

Yes. The model represents atoms rearranging to produce a new compound.

What chemical reactions?

They are reactions involving the breakage of bonds and rearrangement of atoms in reactants to form products.

In the model, the bonds between hydrogen and chlorine were broken. Thereafter, each hydrogen and chlorine molecule linked up to form hydrogen chloride.

Thus, the model shows atoms rearranging to produce a new compound.

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Which of these is a benefit of including comments in your code
makes the app run faster
O helps other programmers understand your code
O improves the user interface
O makes your app more user friendly

Answers

A benefit of including comments in your code is that it helps other programmers understand your code.

What is a Code?

These are numbers and letters which are used to identify a particular thing at a point in  time.

Comments can be put in a code so as to enable others know and understand real intentions about it etc.

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Answer: O helps other programmers understand your code

Explanation: got it right on edgen

what are ways in controlling soil applicable in your community​

Answers

Answer:

Some methods of controlling soil erosion.

1) Planting Vegetation. This method involves planting crops with deep roots that can hold the soil in place. ...

2)Contour Farming. ...

3)Applying Mulches. ...

4)Avoiding Overgrazing. ...

5)Reforestation. ...

6)Use Plastic Sheeting. ...

7)Applying Terraseeding Method

The reaction of a quantity of sulfur trioxide with water gives an acid with a volume of 2 l, containing 1.8 mol of the dissolved oxide.

a)Calculate the mass of ammonium sulfate that would be obtained by reacting with ammonia acid.

b)Calculate the CM of the solution with a volume of 100 ml of ammonia (aq) to be added for complete neutralization of the acid solution.​

Answers

Answer :]

A.)Calculate the mass of ammonium sulfate that would be obtained by reacting with ammonia acid.

Correct me if i'm wrong :]

The picture shows two containers filled with a gas.
Which statement is correct?

The average kinetic energy of the gas particles is greater in container A because it has a lower temperature.
The average kinetic energy of the gas particles is greater in container B because it has a higher temperature.
The gas particles in both containers have the same average kinetic energy because they have the same volume.
The gas particles in both containers have the same average kinetic energy because they have equal number of particles.

Answers

Answer: The average kinetic energy of the gas particles is greater in container B because it has a higher temperature.

What will happenhappen to the pressure of a gas if its Container's volume decreases .

Answers

Answer: The pressure will increase.

Explanation:

    Boyle's law states that "the volume of a gas at constant temperature varies inversely with the pressure exerted on it." In other words, if there is more volume for the gas to spread, the pressure will decrease.

    This also goes the opposite way, meaning with less volume the pressure will increase.

2C4H10+1302 -> 8CO2 + 10H₂O
If 2.46 g of H₂O are produced by a reaction, how many moles of H₂O are produced?

Answers

Answer:

.137 moles

Explanation:

need to know the molar mass of water which is 18.01528 g/mol

2.46 g of H₂O divided 18.01528 g/mol molar mass H₂O = moles of H₂O

2.46 divided by 18.01528 =

.137 moles

C4H10 is butane

butane is commonly used in cigarette lighters

2C4H10+1302 -> 8CO2 + 10H₂O is the burning of butane

burning butane with oxygen makes carbon dioxide and water

vsumhcwagovau

How many moles of aluminum do 6.4×1024 aluminum atoms represent?

Answers

Answer:

11 moles Al

Explanation:

To find the amount of moles, you need to use Avogadro's number to convert the atoms to moles. Since the value you were given has 2 significant figures, the final answer should also have 2 sig figs.

Avogadro's Number:
6.022 x 10²³ atoms = 1 mole

6.4 x 10²⁴ atoms Al                1 moles
----------------------------  x  ------------------------------  = 10.6 moles Al

                                       6.022 x 10²³ atoms

= 11 moles Al

The osmotic pressure of an aqueous solution of ammonium phosphate,
(NH4)3PO4 is 11.4 atm at 27 0C. If the density of this solution is 0.854 g/cm3,
calculate the freezing point the solution. Kf for water is 1.86 0C/m

Answers

Answer:

the freezeng point of solution is -12°c b/c it dencity is 0.854 formula =


In the following acid-base reaction
H₂O is the _

HCI(g) + H₂O(l) →H3O*(aq) + Cl-(aq)

Answers

Answer: base

Explanation:

The water accepted another hydrogen ion, making it a base.

In the given acid-base reaction, H₂O (water) is the base because it accepts a proton (H+) from the hydrogen chloride (HCl) molecule and forms hydronium ion (H₃O+) by gaining an extra proton.

What is an acid- base reaction ?

An acid-base reaction is a chemical reaction in which an acid reacts with a base to form a salt and water. In such a reaction, the acid donates a proton (hydrogen ion) to the base, which accepts the proton. The transfer of a proton from the acid to the base leads to the formation of the conjugate base of the acid and the conjugate acid of the base.

In general, an acid is a substance that donates a proton (H+) and a base is a substance that accepts a proton. When an acid and a base react, the resulting products are a conjugate acid-base pair, where the acid is the product that has gained a proton and the base is the product that has lost a proton.

In the given acid-base reaction, H₂O (water) is the base because it accepts a proton (H+) from the hydrogen chloride (HCl) molecule and forms hydronium ion (H₃O+) by gaining an extra proton.

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A dunk tank hold 30,533 moles of water? How many grams of water are in the tank?

Answers

The mass of water in the tank, given the data from the question, is 549594 g

What is a mole?

The mole of a substance is related to its mass and molar mass according to the following equation:

Moles =[tex]\frac{mass}{molar \;mass}[/tex]

How to determine the mass of water in the tank

From the question given above, the following data were obtained:

Mole of water = 30533 moles

Molar mass of water = 18 g/mol

Mass of water = ?

The mass of the water can be obtained as follow:

Mass = mole × molar mass

Mass of water = 30533 × 18

Mass of water = 549594 g

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What is Matter and give the examples​

Answers

Answer:

Matter is a substance that has inertia and occupies physical space.

Matter is literately in everything

Explanation:

Examples of matter:

Your body

A home

The air

Things that are not matter are:

A light

Heat form fire

Sounds

There not matter because you cant touch, smell or taste them.

Answer:

matter is any substance that occupies space and has weight

Examples

solid,liquids and gas e.t.c

A chemical reaction can be reversed if?

Question 18 options:

The energy of the products is less than the activation energy threshold.


The energy of the products exceeds the activation energy threshold.


The energy of the reactants exceeds the activation energy threshold.


The energy of the reactants is less than the activation energy threshold.

Answers

A chemical reaction can be reversed if the energy of the products exceeds the activation energy threshold.

What are reversible reactions?

Reversible reactions are reactions which can proceed in two directions.

In reversible reactions, reactants can form products and products can also break down to form reactants.

For a chemical reaction to be reversed, the original reaction conditions leading to formation of products are reversed.

Therefore, a chemical reaction can be reversed if the energy of the products exceeds the activation energy threshold.

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Enter your answer in the provided box.

A 4.80−g sample of a salt dissolves in 9.10 g of water to give a saturated solution at 27°C. What is the solubility (in g salt/100 g of H2O) of the salt?

Answers

The solubility (in g salt/100 g of H2O) of the salt is determined as 52.75 g salt.

Solubility of the salt in the water

The solubility of the salt in the given volume of water and temperature is calculated as follows;

4.8/9.1 = x/100

9.1x = 100 x 4.8

9.1x = 480

x = 480/9.1

x = 52.75 g salt

Thus, the solubility (in g salt/100 g of H2O) of the salt is determined as 52.75 g salt.

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Potassium fluoride has a solubility of 92 g of KF in 100. g of H₂0 at 18 °C. Determine if
each of the following mixtures forms an unsaturated or saturated solution at 18 C:

a. adding 24 g of KF to 25 g of H₂O


b. adding 47 g of KF to 50. g of H₂O


c. adding 127 g of KF to 150. g of H₂O

Answers

a) In 25 grams of water, the solubility is 92/4 = 23 grams, so adding 24 grams will produce a supersaturated solution.

b) In 50 grams of water, the solubility is 92/2 = 46 grams, so adding 47 grams will produce a supersaturated solution.

c) In 150 grams of water, the solubility is 92(1.5) = 138 grams, so adding 127 grams will produce an unsaturated solution.

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