use a web search to determine where (in terms of the dna sequence) the restriction enzyme bamh1 cuts the dna.

Answers

Answer 1

The restriction enzyme BamHI cuts the DNA at the recognition sequence 5'-G^GATCC-3', where the caret symbol (^) indicates the site of cleavage.

The restriction enzyme BamHI recognizes and cuts DNA at a specific sequence known as its recognition site. In the case of BamHI, the recognition sequence is 5'-G^GATCC-3'. The caret symbol (^) indicates the site where the enzyme cuts the DNA, specifically between the G and the A in the sequence.

To provide a clearer picture, the sequence 5'-GGATCC-3' is the target site for the BamHI restriction enzyme. Upon recognizing this sequence, the enzyme will bind to the DNA and cleave it between the G and the A, resulting in two DNA fragments.

Understanding the recognition sequence of a restriction enzyme is crucial in molecular biology techniques, such as restriction digestion and DNA cloning. It allows scientists to selectively cleave DNA at specific sites and manipulate genetic material for various purposes, including gene analysis and recombinant DNA technology.

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Related Questions

A research scientist writes a paper on the initial regrowth of a forest after a fire has damaged the entire ecosystem. Which title would be best for the paper?

Answers

The Regrowth after Fire

The title that should be best for the paper should be considered as the Regrowth after Fire

What is an ecosystem?

An ecosystem comprise of the all organism and the physical environment where they could be interacted with each other.

It should be biotic and abiotic component that should be interconnected via nutrient cycles and the flow of energy.

So as per the given situation, the Regrowth after Fire should be considered as the best title.

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how do animals contribute to the carbon cycle

Answers

Answer:

hope it helps have a nice day

Answer: All animals, from humans to the dinosaurs are part of the carbon cycle. When animals eat food, they get carbon in the form of carbohydrates and proteins. The carbon combines with oxygen to form carbon dioxide [CO2] and is released back into the atmosphere as a waste product when animals breathe and exhale.

how does geosphere effect the rock cycle?​

Answers

Hydrosphere and Atmosphere: The erosion of rocks, a major part of the rock cycle and change in the geosphere over time, turns rock into sediment and then, sometimes, to sedimentary rock. ... Different combinations of sedimentary rocks form in environments with different climate conditions.

glutamate is the neurotransmitter released at the rod-bipolar cell synapse. when there is light, the rod membrane potential will

Answers

When there is light, the rod membrane potential will hyperpolarize due to the release of glutamate at the rod-bipolar cell synapse.

In the dark, rods are depolarized and continuously release glutamate onto bipolar cells, inhibiting their activity. When light enters the eye and is absorbed by the rod photoreceptor cells, a cascade of events occurs, leading to the closure of sodium channels and hyperpolarization of the rod membrane. This hyperpolarization reduces the release of glutamate from the rod cells onto bipolar cells.

With decreased glutamate release, bipolar cells become disinhibited and can transmit signals to downstream cells in the visual pathway. Therefore, in the presence of light, the rod membrane potential will hyperpolarize, resulting in a reduction of glutamate release at the rod-bipolar cell synapse.

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which statement best describes a cause and its effect during the process or muscle contraction?​

Answers

Answer. The most appropriate statement that is explaining the effect and cause during muscle contraction is. “The release of calcium ions causes myosin and actin to attach to each other.” The activation of muscles helps in the generation of muscles that send out the signals to the neurons.

hope this helps

Which pair of microscope views represents a eukaryotic cell and prokaryotic cell?

Answers

Answer: it might Be C or B

Explanation:

8th grade science plsss answer

Answers

Answer:

Type the question

Explanation:

I can't see

Answer:

me neither

Explanation:

How do you suppose changes in DNA lead to speciation

Answers

Answer:

The correct answer is - change in DNA is called mutation which is essential for evolution including speciation.

Explanation:

A mutation is a change in the genetic or hereditary material which is DNA. A mutation is the main reason behind the evolution of the organism or species and speciation can be led by evolution, which takes place by results in the accumulation of many small changes in DNA or genetic changes in a population over a long period of time.

Mutations that change in DNA are required for the evolution process they are the raw material of genetic variation. without changes in hereditary material, there is no speciation or evolution possible.

Which of the following best explains why ligase is required for DNA replication?
Ugase facilitates the binding of RNA polymerase to the promoter region.
Ligase enables the newly synthesized DNA to twist into a double helix
Ligase forms the hydrogen bonds between complementary bases in the two strands of DNA
The lagging strand cannot be replicated continuously, and ligase is needed to join the fragments

Answers

The lagging strand cannot be replicated continuously, and the need for ligase to join the fragments best explains why ligase is necessary for DNA replication. Here option D is the correct answer.

DNA replication is the process by which a cell duplicates its DNA prior to cell division. It involves the synthesis of two new strands of DNA, the leading strand, and the lagging strand. The leading strand is synthesized continuously in the 5' to 3' direction, whereas the lagging strand is synthesized in short fragments called Okazaki fragments.

The lagging strand is synthesized in the opposite direction of DNA unwinding, making it necessary to synthesize it discontinuously. As the replication fork progresses, short Okazaki fragments are synthesized in the 5' to 3' direction away from the replication fork. However, these fragments are not yet connected to form a continuous strand.

This is where ligase comes into play. Ligase is an enzyme that plays a crucial role in DNA replication by catalyzing the joining (ligation) of the Okazaki fragments on the lagging strand. It forms phosphodiester bonds between adjacent nucleotides, sealing the gaps between the fragments and creating a continuous, double-stranded DNA molecule.

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Complete question:

Which of the following best explains why ligase is required for DNA replication?

A - Ugase facilitates the binding of RNA polymerase to the promoter region.

B - Ligase enables the newly synthesized DNA to twist into a double helix

C - Ligase forms the hydrogen bonds between complementary bases in the two strands of DNA

D - The lagging strand cannot be replicated continuously, and ligase is needed to join the fragments

Prokaryote regulate gene expression at which of the following levels?
1. mRNA transcription
2. mRNA translation, mRNA translation and epigenetic inheritance
3. epigenetic inheritance only
4. mRNA translation and epigenetic inheritance
5. alternative mRNA processing and chromatin compaction.
Please help to choose the best answer.

Answers

Prokaryotes regulate gene expression at 1. mRNA transcription level.

Prokaryotes largely control mRNA transcription when it comes to controlling gene expression. Prokaryotic gene regulation entails regulating the start of transcription by enhancing or impairing RNA polymerase's affinity for the promoter region of the gene. The binding of transcription factors, presence of activators and repressors, and presence of certain DNA sequences are only a few of the techniques used to achieve this control.

Prokaryotes also have other levels of control of gene expression, including as mRNA translation, mRNA processing, chromatin compaction, and epigenetic inheritance, although these levels are not as important as mRNA transcription in terms of primary regulation. These extra degrees of control over gene expression are more typical of eukaryotic cells.

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which of the following element is not present in proteins

Answers

Answer:

Protein: a compound made of amino acids. Each amino acid is made of carbon, hydrogen, oxygen, and nitrogen. Most proteins also include sulfur.

Which means the only choice left is helium

i hope this is the answer you are looking for


28. Eubacteria can be distinguished from archaebacteria by
a.The presence of a true nucleus
b. Being multicellular
Difference in the makeup of cell wall
d. The presence of organelles

Answers

Answer: D

Explanation:

i am sorry if this is wrong

Which of the following is a correct representation of secondary
succession?


A. bare rock -> lichens + mosses -> grass -> shrubs -> trees

B. trees -> shrubs
-> flowers -> grass

C. soil -> grass -> shrubs -> trees

D. grass -> bare rock -> soil -> trees

Answers

The answer is b because it’s tree for free shrubs

Digestive enzymes in your stomach and intestines help digest the food you eat. If you are lactose intolerant and cannot digest milk products, it is the result of a mutated message in your...

Group of answer choices

lysosomes

nucleolus

chromosomes

X and Y chromosome

Answers

Answer:

chromosomes

Explanation:

that is what your genes are located in

28)
What is a molecule made from joining together small molecules called monomers?
A)
amino acid
B)
glucose molecule
C)
nucleotide
ne
D)
polymer
20

Answers

Answer: D)

polymer

Explanation:

Just a random question cuz why not? Brainliest to whoever answers correctly!

What is the difference between biology and chemistry?

Answers

Answer:

Biology is science about life and how it survives, breeds, and dies,

Whereas chemistry is about chemical concoctions such as sulfuric acid.

This is what I THINK lol
Biology- plants and human body
Chemistry- elements and reactions that make up and result in plants and the formation of the human body

What do organisms use most of this energy for?
a) so they can continue to digest food
b) so they can continue to move around
c) so they can continue to breathe
d) so they can grow repair and reproduce
e) all of the above​

Answers

Answer:

E

Explanation:

They need energy to do all these necessary feats.

All of the above. hope this helps!!

Help please <3 Thank you

Answers

Answer:

The answer is C

:::::::::::::::::::

How do plants obtain their energy?

Answers

Answer:

they obtain their energy from the sun and water and pollen

Explanation:

Sorry if I'm wrong have a good one!

Why are Wind Turbines being moved offshore?

Answers

Answer:

Wind turbines are being moved offshore because the wind comes at the right time: Offshore winds are usually higher throughout the day, allowing for more reliable and productive electricity output when market demand is highest. The majority of land-based wind resources are more efficient at night when demand for electricity is smaller.

Select all of the following that are true regarding mutations.
a) All mutations are harmful
b) Some mutations have little to no effect on protein folding
c) A mutation that drastically changes a protein is likely to be beneficial
d) Mutations can be the cause of diseases
e) Mutations are alterations in DNA sequences

Answers

The true statements regarding mutations are: Mutations can be the cause of diseases and Mutations are alterations in DNA sequences (Option D, E).

Mutations are defined as changes or alterations in the DNA sequence of a gene or chromosome. They can occur spontaneously or as a result of exposure to certain substances (mutagens) or radiation. Mutations can result in a change in the protein that is made by a gene. The two true statements regarding mutations are Mutations can be the cause of diseases and Mutations are alterations in DNA sequences. Some mutations have little or no effect on protein folding, but others can result in the creation of a non-functional protein or one that does not perform as it should. Mutations that drastically change the protein are unlikely to be beneficial because they can result in the protein losing its function or in a harmful function. Therefore, mutations can cause diseases.

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(c) Based on the data, describe the time period during which Chlorella approximates exponential growth. Based on
the data and assuming logistic growth, describe the time period during which the Chlorella population has reached
the carrying capacity of the culture. Based the data, calculate the growth rate per day for the 5-day period with
the greatest growth rate.

Answers

Populations that experience a logistic growth model depend on the density and available resources. A) 5 - 10 days. B) 25 - 30 days. C) 0.27 x 10⁶cells/mL/day

What is the logistic growth model?

In a logistic growth model, the population growth depends on density. Natality and mortality depend on the population size, meaning that there is no independence between population growth and population density.

When a population grows in a limited space, density rises gradually and eventually affects the multiplication rate. The population per capita growth rate decreases as population size increases.

The population reaches a maximum point delimited by available resources, such as food or space. This point is known as the carrying capacity, K.

K is a constant that equals population size at the equilibrium point, in which the natality and the mortality rate get qual to each other.

Assuming that the population size is N, when

N<K, the population can still grow. N approximates to K, the population's growth speed decreases. N=K the population reaches equilibrium, N>K, the population must decrease in size because there are not enough resources to maintain that size.  

The sigmoid curve represents the logistic growth model.

In the example,

Between 0 and 5 days the population remains relatively stableBetween 5 and 10 days Chlorella approximates exponential growth.Between 10 and 20 days Chlorella experiences exponential growth. Between 20 and 25 days the population is near the K and decreases its growth rate.From 25 to 30 days, the population reached the equilibrium point, and got to stabilize.  

Time period during which Chlorella,

          A) approximates exponential growth ⇒ Between 5 and 10 days

Between 10 and 20 days its already exponential growth.

         B) has reached the carrying capacity of the culture ⇒ 25 to 30 days

Finally,

        C) Growth rate per day for the 5-day period with the greatest growth rate.

First we need to figure out during which period Chlorella experiences the  greatest growth rate. To do it, we just need to make the following substraction,

Growth rate per period = final concentration - initial concentration

Period 0-5 ⇒ 0.08 - 0.01 = 0.07Period 5-10 ⇒ 0.55 - 0.08 = 0.47Period 10-15 ⇒ 1.9 - 0.55 = 1.35Period 15-20 ⇒ 2.8 - 1.9 = 0.9Period 20-25 ⇒ 3.2 - 2.8 = 0.4Period 25-30 ⇒ 3.2 - 3.2 = 0  

Now we know that during the period from 10 to 15 days, the population experienced its greatest growth rate. But this rate is acchieved in 5 days. Now we need to find out the growth rate per day. So all we need to do, is to divide the result of the substraction per 5 days,

1.35 x 10⁶ / 5 days = 0.27 x 10⁶ cells/mL/day

The answer is 0.27 x 10⁶ cells/mL/day

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If two or more elements chemically combine what do they make?

Answers

chemical compound i think

5) Describe crossing-over. When does crossing-over occur? What is the result?

Answers

Answer:

Crossing over occurs between prophase I and metaphase I and is the process where two homologous non-sister chromatids pair up with each other and exchange different segments of genetic material to form two recombinant chromosome sister chromatids.

Explanation:

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How could you describe the levels of organization in a decomposer?

Answers

an organism, especially a soil bacterium, fungus, or invertebrate, that decomposes organic material.

How did beadle and tatum’s work on auxotroph’s suggest that metabolism was controlled by protein enzymes?

Answers

Beadle and Tatum's work on auxotroph's suggested that metabolism was controlled by protein enzymes by identifying the link between genes and enzymes. This theory is also referred to as "one gene, one enzyme hypothesis" as stated in their experiment.

The experiment was conducted using a bread mold called Neurospora crassa, where they grew the mold on a minimal growth medium that lacked specific nutrients. The scientists observed that certain mutations in the mold led to the inability to grow on this minimal growth medium. They identified the mold that could grow on a medium containing arginine, but failed to grow on a medium that lacked arginine. They then discovered that the mutated molds were deficient in producing specific enzymes required to produce arginine and could only grow on a medium containing arginine.Since the mutants required a specific nutrient that the wild types did not, Beadle and Tatum postulated that the mutations were caused by altered or disrupted genes that affect the enzymes produced by these genes.

Thus, the mutants were unable to produce the required enzymes, which resulted in their inability to grow on the minimal growth medium. Therefore, the experiment concluded that one gene encoded for one enzyme which was responsible for controlling the metabolic pathway of producing arginine. The experiment showed that each gene encoded for a specific enzyme in the metabolic pathway that controlled the production of arginine.

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Which term best describes the number of calories the body uses at rest?
A. basal metabolic rate
B. resting caloric count
C.resting heart rate
D. body mass index

Answers

The correct option is (a). The term that best describes the number of calories the body uses at rest is basal metabolic rate (BMR).

The term that best describes the number of calories the body uses at rest is basal metabolic rate (BMR). BMR is the amount of energy that your body requires to maintain basic functions like breathing, circulation, and maintaining body temperature while you are at rest. It's influenced by factors such as age, gender, weight, body composition, and genetics. Basal metabolic rate (BMR) is a measure of how many calories the body uses when at rest. The basal metabolic rate is the minimum number of calories the body requires to sustain its vital functions, including heartbeat, respiration, and maintenance of body temperature, while at rest. This is typically measured while you are awake, but relaxed and in a fasting state. Basal metabolic rate (BMR) refers to the amount of energy that your body requires to maintain basic functions while at rest, and it is influenced by several factors. BMR accounts for around 60-70% of your total daily energy expenditure, which means that it plays a significant role in weight management. For instance, if you have a high BMR, you will burn more calories even when you are not active, which can help you lose weight or maintain a healthy weight. However, if you have a low BMR, you will burn fewer calories even when at rest, which can make it harder to lose weight.To calculate BMR, one needs to take into account their age, gender, weight, height, and body composition. The formula used to calculate BMR is different for men and women.

For instance, for men, the formula is BMR = 88.362 + (13.397 x weight in kg) + (4.799 x height in cm) - (5.677 x age in years).

For women, the formula is BMR = 447.593 + (9.247 x weight in kg) + (3.098 x height in cm) - (4.330 x age in years).

Knowing your BMR can help you determine how many calories you need to consume daily to maintain your weight or achieve your weight loss goals.

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Division creates 2 types of B Cells: _________________ and Memory B Cells

Answers

Lymphocyte is the anwer

Answer:

Hello There!!

Explanation:

The answer is plasma cells.

hope this helps, have a great day!!

~Pinky~


Question 4
Blood sugar homeostasis plays an important role in our health and well-being. If
blood sugar levels get too low or high, there are serious health complications that
can result. Which two body systems regulate blood sugar levels? Click on the correct
answers.

Select 2 correct answer(s)

A-Nervous System
B-Musculoskeletal System
C-Respiratory System
D-Digestive System

Answers

Nervous and digestive system

Which type of reproduction produces offspring with more genetic variations?
(Include the terms "mitosis" and "metosis" in your answer)

Answers

Answer: Meiosis

Explanation:

Mitosis results in clones that are genetically identical to their parents. Meiosis has processes like crossing over and the law of independent assortment which allow for genetic variatoin and recombinants.

Other Questions
Select the correct text in the passage.One theme in this excerpt from Isaac Asimovs The Fun They Had is remembering the past. Which sentence in the excerpt best reflects this theme?Margie went into the schoolroom. It was right next to her bedroom, and the mechanical teacher was on and waiting for her. It was always on at the same time every day except Saturday and Sunday, because her mother said little girls learned better if they learned at regular hours.The screen was lit up, and it said: "Today's arithmetic lesson is on the addition of proper fractions. Please insert yesterday's homework in the proper slot."Margie did so with a sigh. She was thinking about the old schools they had when her grandfather's grandfather was a little boy. All the kids from the whole neighborhood came, laughing and shouting in the schoolyard, sitting together in the schoolroom, going home together at the end of the day. They learned the same things, so they could help one another on the homework and talk about it.And the teachers were people. . . .The mechanical teacher was flashing on the screen: When we add the fractions and Margie was thinking about how the kids must have loved it in the old days. She was thinking about the fun they had. Which is the correct stem-and-leaf plot for the data set?16, 15, 47, 41, 40, 39, 16, 37 Which action is an example of the United States using economic influence asa tool of foreign policy? please help due today The term used to describe how capable and significant a person feels; it is typically a key contributor to emotional well-being. It is used to answer the question, "What do I believe about myself?self-appraisalself-concpetself-esteemself-efficacy 4 reasons why we should apply tertiary early Which of the following is not a diagnosable disorder according to the DSM-5? oGeneralized anxiety disorder. oSex addiction. oTrichotillomania. oSexual masochism Explain how you would graph the inequality: 3 x use a reference angle to write sec290 (where the angle is measured in degrees) in terms of the secant of a positive acute angle. do not include the degree symbol in your answer. La influencia que tiene la temperatura en la reproduccin de los paramecios, en muestras de agua estancada which is the correct range for the temperature danger zone? question 2 options: 0-32f 40-100f 40-140f 100-200f What percent of a day does this child spend doing homework? (Round to the nearest whole percent) What causes wave-cut cliffs to form? please explain every step it's related about tripleintegral and cylinderical coordinates2. Set up the triple integral in cylindrical coordinates to integrate the function f(x, y, z)=(2x' +2y+)over the solid region D that is inside the cylinder x + y =9, below the plane z=-x+4, and above Marissa has a yard service to help people in her neighborhood. She earns $15 for each lawn she mows, $10 for each yard she weeds, and $5 for each yard she rakes. This month Marissa spent $3 on flyers to send out to neighbors, she purchased a new blower for $26, and paid her brother $18 for helping her mow lawns. If Marissa mowed 4 lawns and raked 8 yards, how much money did she make in profit? 1. 53 2. 100 3. 36 4. 71 . ___________________ is the process of plants releasing oxygen into the environment? Select the correct answer.Which statement correctly compares the graph of function g with the graph of function ??FE) = e-g(x) = - 4O A.OB.O C.O D.The graph of function g is a horizontal shift of the graph of function to the right.The graph of function g is a vertical stretch of the graph of function f.The graph of function g is a vertical compression of the graph of function f.The graph of function g is a horizontal shift of the graph of function f to the left.ResetNext Which transitional word or phrase is most appropriate for an essay that is organized chronologically?Next,All in all,On the other hand,However, 8 divided by 618 .............. Let W = {a + 2x + bx^2 P2 : a, b R} with the standard operations in P2. Which of the following statements is true? A. W is not a subspace of P2 because 0 W. The above is trueB. W is a subspace of P2. The above is true C. None of the mentioned D. 1+xEW