We will be working with Escherichia coli, a bacteria that requires oxygen for growth. Very briefly describe the conditions needed to grow 5ml of liquid culture of E.Coli in the laboratory?

Answers

Answer 1

Answer:

The conditions required to grow of liquid culture of E. coli includes various aspects such as temperature, equipment, media, oxygen, and glassware. These aspects are describe as per requirement below:

1. Suitable temperature for E. coli is higher than room temperature should be maintain at 37 degree Celsius as it is inhibits the bacteria growth.

2. Autoclave helps in regulating conditions during growing period of bacteria whereas Laminar air flow provides sterilized environment during culturing or sub culturing E. Coli colony.

3. LB medium is suitable for liquid medium of E.coli which requires peptides, vitamins, trace elements and minerals and digested food as nutrients.

4. It is anaerobic bacteria so oxygen is not essential for culture however can grow in availability of oxygen.

5. Properly sterilized glassware obtained by placing it in Bunsen Burner's flame which is essential to avoid contamination.


Related Questions

-Give an example of a scientific law.
-Give an example of a theory in science.
Please help :)

Answers

Answer:

An example of a scientific law is Newton's first law.

Explanation:

It says that every object will remain at rest or in uniform motion in a straight line unless forced to change direction by the action of an external force.

An example of a theory in science is the Big Bang theory. It is something that someone came up with that is a rational explanation to explain why or how something happened or came into place.

How could you model this process in a different way? Explain how you’d design this model so that the entire process is clear to the reader.

Answers

Answer:

Edmentum and Plato sample answer

Like and Rate

An illustrated diagram would be helpful in understanding the transmission. The diagram would show an adult, with the major parts of the nervous system highlighted. The nerve pathways involved in stimuli processing and response would be highlighted in different colors to show autonomic and somatic and parasympathetic and sympathetic parts of the nervous system.

What organelle creates rRNA for synthesizing ribosomes?

Answers

Answer:

nucleus

Explanation:

The nucleus contains the cell 's DNA and directs the synthesis of ribosomes and proteins.

Answer:

nucleolus

Explanation:

whos trying to helps me ?

Answers

Answer:

1.9mL to 8.7mL (Answer A)

Answer:1.9ml to 8.7ml

Explanation:

Suppose you have two liquids. One has a pH of 2. The other has a pH of 9. What can you tell from those pH?

Answers

Answer:

The liquid with a pH of 2 is an acid. The liquid with a pH of 9 is a base.

Explanation:

pH Scale:

1    2    3    4    5    6    7    8    9    10    11    12    13    14

A pH scale ranges from 1 - 14.7 is neutral.Any number on the scale less than 7 is acidic (acid).Any number on the scale more than 7 is basic (base).

In what form does carbon move from the atmosphere into the biological
food webs?

Answers

Answer:

Carbon enters all food webs, both terrestrial and aquatic, through autotrophs, or self-feeders. Almost all of these autotrophs are photosynthesizers, such as plants or algae. Autotrophs capture carbon dioxide from the air or bicarbonate ions from the water and use them to make organic compounds such as glucose.

Explain in detail the inter-relationship
between the food chain and food
web.​

Answers

Answer:

A food web consists of many food chains. A food chain only follows just one path as animals find food. eg: A hawk eats a snake, which has eaten a frog, which has eaten a grasshopper, which has eaten grass. A food web shows the many different paths plants and animals are connected.

11. Using the diagram to the left,if line n bisects QR find QP

Answers

Answer:

QP = 41

Explanation:

Its geometry its easy...

Since its getting cut through the middle that means they equal each other

3x + 5 = 5x - 19 (Use a calculator)

x = 12

Since your solving to find QP you plug it into QP

3(12) + 5 = 41

:)

is Wood Block a mineral or non-mineral

Answers

Answer:

Non-mineral

Explanation:

Wood is not a mineral. Minerals are rock and other types, but wood is not a rock.

select the correct answer from each drop down menu. BLANK will develop pharyngeal arches. BLANK will develop a postnatal tail in the embryonic stage.

the first blank the opinions are
1. earthworms
2. barb fish
3. crabs
4. leeches

and for the second the opinions are
1. ladybugs
2. snails
3. sea cucumbers
4. newts

Answers

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Actually Welcome to the Concept of the Biology

1) Brab Fish

2) Newts

Write one scientific question about the organism in the photo.

Answers

Answer:

why would this organism a close ancestor of humans evolve differently than us.

What is the importance of studying fossils? * A. To provide information about current life forms and current environmental conditions B. To provide information about how life and environmental conditions may have changed C. To provide information about how previous life forms would exist in current environmental conditions D. To provide information about how current life forms would exist in previous environmental conditions

Answers

Answer:

The answer D

Explanation:

Paleontologists find and study fossils all over the world, in almost every environment, from the hot desert to the humid jungle. Studying fossils helps them learn about when and how different species lived millions of years ago. Sometimes, fossils tell scientists how the Earth has changed.

Which sphere was impacted the most?

Answers

Humans are part of the biosphere because we are living things. The biosphere includes ALL living things.

The component of the hydrosphere most greatly affected by this emission of carbon dioxide is the ocean. carbon cycleThe generalized carbon cycle.

Describe how the following are important in the makeup of the cell membrane: 1. Phospholipid bilayer 2. Proteins 3. Carbohydrates 4. Cholesterol

Answers

Answer:

1. Phospholipid bilayer - The lipid bilayer acts as a barrier to the passage of molecules and ions into and out of the cell

2. Proteins - Membrane transport protein (or transporter) is a kind of membrane protein that involved in the movement of ions, small molecules, or macromolecules across a biological membrane.

3. Carbohydrates - Carbohydrates covalently linked to proteins (glycoproteins) or lipids (glycolipids) are also a part of cell membranes, and function as adhesion and address loci for cells.

4. Cholesterol - Cholesterol reduces permeability of lipid membranes. ... Cholesterol plays has a role in membrane fluidity but it's most important function is in reducing the permeability of the cell membrane.

Explanation:

What is a trophic cascade?

Answers

Trophic cascades are powerful indirect interactions that can control entire ecosystems
They are powerful interactions that can effect the world such as global warming

Which describes a group of organisms that are members of a species?

Answers

Answer:

Population is defined as a group of organisms, which belong to the same species. Example- Human population includes a group of organisms that all belong to the species sapiens

Explanation:

Describe the function of each organelle.
Nucleus

Answers

awnser:

The main function of the cell nucleus is to control gene expression and mediate the replication of DNA during the cell cycle. The nucleus is an organelle found in eukaryotic cells. Inside its fully enclosed nuclear membrane, it contains the majority of the cell's genetic material.

Answer:

The nucleus is the central and most important part of an object, it controls and regulate the activity of cells. The nucleus also carries the genes.

Explanation:

Urgent I’m so confused
Homeostasis is when an internal stimulus causes an external response.
True or False???

Answers

True. Both internal and external stimuli can cause a response or resulting reaction in a organism.
True. Hope this helps!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

Why were Oparin and Darwin's ideas about the Origins of Life not immediately considered to be part of a scientific theory about the origins of life on Earth?

Answers

Answer:

Darwin's opinion seems to have changed over time from his original remark in the 1861 3rd edition of The Origin of Species.

Oparin and Darwin’s ideas about the Origins of life are not immediately considered to be part of a scientific theory about the origins of life on earth because the ideas they proposed were difficult to test or observe.

What is Oparin's hypothesis regarding the origin of life?

According to the Oparin hypothesis life originate from inorganic molecules, like amino acids considered to be formed first, and then combines to form complex polymers.

This hypothesis has no scientific proof due for this reason the idea of Oparin idea was not immediately considered to be part of a scientific theory.

The theory given by Darwin challenged the idea that God is the creator of all animals and plants that are present on earth and there were no proper pieces of evidence to prove this theory when it is published to convince several scientists.

Due to a lack of pieces evidence and scientific proof, Oparin and Darwin's ideas about the Origins of Life on earth are not immediately considered to be part of scientific theory.

Learn more about Origin of Life, here:

https://brainly.com/question/13185637

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What trophic levels contain more energy? please help

Answers

Answer- Since the source of energy is the sun, the trophic level representing producers (plants) contains the most energy.

Which of the following is qualitative information?

A. The mineral is red and hard
B. The mineral has a hardness of 8
C. The mineral has a mass of 2 grams
D. The mineral has a volume of 4 cm3

Answers

Answer:

option A. is correct.

hope it helps

Answer:

 A, The Mineral is red and hard

Explanation:

    Because the definition of qualitative is describing an object based on looking at it, and the other selections are just weight and feel.

              Hope This Helped

______ is a part of all organic compounds, which make up living things. ​

Answers

Answer:

Carbon.

Explanation:

Carbon is a part of all organic compounds.

how do we know energy is transferred in an energy pyramid?​

Answers

Answer:

While food chains and food webs show relationships between organisms, and energy pyramid quantifies the amount of energy transferred from one trophic level to the next, when one organism eats another, wherein approximately 10% of the energy available is retained and passed along.

You are a virologist interested in studying the evolution of viral genomes. You are studying two newly isolated viral strains and have sequenced their genomes. You find that the genome of strain 1 contains 25% A, 55% G, 20% C, and 10% T. You report that you have isolated a virus with a single-stranded DNA genome. Based on what evidence can you make this conclusion

Answers

Answer:

See the answer below

Explanation:

In double-stranded DNA, the ratio of purine bases (Adenine and Guanine) and that of pyrimidine bases (Thymine and Cytosine) is 1:1 according to Chargaff's rule. This means that the percentage of Adenine must be equal to that of Guanine while that of Thymine must also be equal to Cytosine.

However, in the case of the newly isolated viral strain, the amount of Adenine (25%) and Guanine (55%) vary while that of Thymine (10%) and Cytosine (20%) also vary. This is in contrast to Chargaff's rule. Hence, the isolated virus is a single-stranded DNA virus instead of a double-stranded.

Why is water important for digestion

Answers

Water is essential for good health. Water and other liquids help break down food so that our body can absorb the nutrients. Water also softens stool,which helps prevent constipation.

name the following the plant hormone which is responsible for the ripening of fruits​

Answers

Answer:

ethylene

Explanation:

plant hormone responsible for fruit ripening as well as senescence of vegetative tissues.

In 1935, Crocker proposed that ethylene was the plant hormone responsible for fruit ripening as well as senescence of vegetative tissues.

Only green organisms have chloroplasts, so do green frogs and toads have chloroplasts??

Answers

Answer:

Chlorophyll is a cell that comes from plants not animals.

Explanation:

Chlorophyll is the part of chloroplast and is the light absorbing pigments which provide green color to the plants, but chloroplast traps the solar energy, which is the site of photosynthesis and other chemical reactions and works as the ‘powerhouse of the cell’ like mitochondria.

explain why it is important for a scientist to be both logical and creative.

Answers

Because the way of using logic and creativity can help expand your mind through more ideas and facts. As your knowledge grows bigger.
well think of it like this, doctors have to think creatively sometimes in order to find ways to fix their patients problems right ? well then that would also show that scientists need to be creative when thinking of ways to make new medicines, deconstruct elements and make new compounds. Their creativity along with their logical knowledge of the task at hand gives them ample opportunity to create new ways to solve their problems. How do you think most pharmaceuticals were developed?
( hope this helps (: )

NEED HELP ASAP FIRST PERSON TO CORRECTLY ANSWER WILL GET MARKED BRAINLIEST
4. What statement best describes the composition (parts that make up) of an
ecosystem?
a. Animals and rocks
b. Bacteria and fungi
C. Sunlight and water
d. Biotic and abiotic factors

Answers

Answer:

D

Explanation:

This includes all things living and not.

C, because an ecosystem can be categorized into its abiotic constituents, including minerals, climate, soil, WATER, SUNLIGHT. :)

is suger a monomer and suger cube a polymer?​

Answers

sugar is a monomer called monosaccharides and sugar cubes are polymer because a polymer has a molecular structure
Other Questions
Match each descriptive label with the correct excerpt from Britten's Young person's Guide to Orchestra. Select the statements that correctly describe buffers. A) A buffer is made up of a strong acid and a strong base. B) The pH of a buffer solution does not change significantly when a small amount of acid is added. C) The pH of a buffer solution is determined by the ratio of the concentration of acid to the concentration of base. D) The K_a of a buffer decreases slightly when a small amount of acid is added to the buffer solution. E) A strong acid added to the buffer solution reacts with the weak acid of the buffer. What is a medium? Wanna make sure I got it right before it gets turned in.Need asap. Which of the following equations can be used to solve volume of a solution usingmolarity equation? Which was the earliest published book? Type your answer in below! (Stephen King books btw) Differentiate between a pure substance and a mixture and provide examples can someone please simply teach me what mean, median, and mode is pls pls pls 1. Simplity the following into lowest term 3x - 2 - 2x Help me plz science middle school Which of the following is an appropriate hypothesis to answer the question, why are leaves green?Based upon the given data, which spans 25 years of research, scientists determined that leaves are grefound within leaves.Using the work of previous scientists and adding new data to their information, scientists have found topigmented compound found within leaves.To study the coloring of leaves, scientists grew 250 maple trees within a greenhouse over the courseLeaves are green due to the presence of chlorophyll, a pigmented compound found within the leaves Which two advantages do renters have that home buyers dont have? In fluorescence microscopy, the _____ absorbs light energy from the excitation light and emits colored light. -What factors determine an individuals ethics. For you, which factor has been the most significant? How would you go about simplifying 5,759 over 999? Based on this passage,the scarlet ibis is a symbol of both idfwuomg1 5000+25000 Tickets to the movies are $7 for adults and $4 for children. If 272 ticketssold $1694, then how many of each type were sold? Tais baby brother weighs 3,745 grams. What is his weight in kilograms? A. 0.3745 kg B. 3.745 kg C. 37.45 kg D. 374.5 kg A graph of the Soaring Cupcakes cost per cupcake is shown below. The graph shows a relationship between the number of cupcakes purchased, n, and the total cost of the order, t. The Soaring Cupcake charges a $15 initial fee and then $3.00 for every cupcake purchased. What is the total cost for 15 cupcakes? What is the total cost for 10 cupcakes? How many cupcakes can you order for $30? Write a function rule in function notation for the situation using the variables t and n: A.) Does an input value of 4 make sense for this situation, why? B.) Does an input value of 5.2 make sense for this situation, why? In all society, the government decides what to produce? True OrFalse?